If you are directly affected by any of the recent hurricane activity impacting the western hemisphere and were not able to register before the extended deadline, please contact the AMP Education at ampeducation@amp.org.


The AMP 2017 Molecular Pathology Outreach Course (MPOC) is a live event held the day before the Annual Meeting. This course is designed for pathologists, residents, laboratory directors and technologists who have some prior exposure to molecular diagnostic testing. The course features introductory information about the state of the science as well as a series of case studies illustrating some of the most current principles and practices in the field of Molecular Pathology. Attendees from all career levels will benefit from this staple offering.


Visit the Program page for more information.



Wednesday, November 15, 2017 | 8:30am - 3:45pm

Location: Salt Lake Marriott Downtown at City Creek, Salt Lake City, UT

Presented by the AMP Training & Education Committee


How to Register:

  1. Under the "Products" menu, add the course to your basket.
  2. Login with your AMP Member/Customer Username and Password. (If you require your user name, please send an e-mail containing your first and last name to amp@amp.org.
    Your user name will be sent to the preferred e-mail address designated on your AMP profile.)
  3. Proceed to "Checkout."
  4. Provide your payment information by clicking “Edit my Billing Info”.
  5. Save your billing information and click “Submit Order.”
  6. You will receive a confirmation email from Peach New Media.
  7. Closer to the event, you will receive an email from ampeducation@amp.org with more information about the course and accessing the course materials.


* If you have any dietary restrictions or other special needs, please email meetings@amp.org.


Registration Rates


Early Rates

Ends: 9/15/17

Advance Rates

Ends: 10/21/17

Onsite Rates

After 10/21/17

AMP Regular Members


$325 $375
AMP Technologist Members $185 $235 $285
AMP Associate Members $185 $235 $285
Non-AMP Members $300 $350 $400

If you are required to pay by check, please download, complete, and return the AMP MPOC 2017 Registration Form.


CE Credit



This activity ("Association for Molecular Pathology 2017 Annual Meeting") was planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education through the joint providership of American Society for Clinical Pathology (ASCP) and the Association for Molecular Pathology (AMP). The ASCP is accredited by the ACCME to provide continuing medical education for physicians.



The ASCP designates this live educational activity for a maximum of 4.5 AMA PRA Category 1 Credit(s)TM. Physicians should claim only the credit commensurate with the extent of their participation in the activity.


This continuing medical laboratory education activity is recognized by the ASCP for up to 4.5 hours of CMLE credit. ASCP CMLE credit hours are acceptable for the ASCP Board of Certification (BOC) Certification Maintenance Program (CMP).


SAM Credit

This activity ("Association for Molecular Pathology 2017 Annual Meeting") is approved by the American Board of Pathology. Physicians should only claim credit commensurate with the extent of their participation in the activity. Participants must successfully complete the online exam (answering at least 80% of the questions correctly).

After the course, you will be given access to the SAM online exam and CME/CMLE online evaluation.



Cancellation Policy


Cancellations must be received in writing to (ampeducation@amp.org) no later than October 20, 2017. A $75 processing fee will be applied. Absolutely no refunds after October 20, 2017.


Photography Disclaimer

Registration and attendance at or participation in AMP meetings and other activities constitutes an agreement by the registrant to allow AMP to use and distribute the registrant or attendee's image or voice in photographs, videotapes, electronic reproductions, and audiotapes of such events and activities.


Course Objectives
  • Compare and contrast a number of commonly utilized "ready out of the box" platforms with regard to their non-template amplification methodology and their clinical utilization;
  • Enumerate several technological and clinical adaptions for PCR;
  • Discuss the methodologic and clinical considerations of cell free DNA testing;
  • Compare and contrast methods of single nucleotide variant detection, copy number detection, and structural variant (fusions) detection by NGS and other related platforms (for CNVs).
Course Information
Date Presented:
November 15, 2017 12:00 AM Eastern
No Longer Available
Click on a topic name to see details and purchase options.
Wednesday November 15, 2017


Federico A Monzon, MD
Castle Biosciences

This presentation provides an overview of major clinically used molecular diagnostic tests emphasizing the importance of quantitative and qualitative sample requirements as a prerequisite for successful testing. A growing menu of molecular tests may require adjustment of sample triage and processing algorithms to optimize the use of materials. Pathologists play a crucial role in selecting appropriate specimens and ensuring its adequacy, particularly in solid tumor testing.

- Compare main types of samples submitted for molecular testing highlighting key criteria for specimen selection.

- Assess requirements for the sample adequacy in relation to the test performance characteristics.Speaker:

Speaker: Anna Yemelyanova, MD University of Texas MD Anderson Cancer Center

Cleavase Probe Amplification for the Factor V (F5) and Prothrombin (F2) Genes


Mutations in the Factor V and prothrombin genes have been implicated in deep vein thrombosis. A case presentation will illustrate the application of the cleavase probe amplification method in the detection of these mutations and their clinical implications.

- Explain the mechanism of the cleavase probe signal amplification methodology.

- Describe the application of testing for genes involved in thrombophilia.

Speaker: Cynthia Jackson, PhD Rhode Island Hospital


Finding What\\\'s Lost: Multiplex Ligation-Dependent Probe Amplification (MLPA) for Detection of Large Deletions in Cystic Fibrosis


This case covers the basics of the MLPA method for detection of copy number variants. A brief review of Cystic Fibrosis (CF) and CF carrier screening will be presented with a specific case to illustrate how MLPA can be clinically applied in challenging cases to reach an appropriate diagnosis.

- Explain the mechanism of the cleavase probe signal amplification methodology.

- Describe the application of testing for genes involved in thrombophilia.

Speaker: Mark Ewalt, MD University of Colorado

HPV Genotyping Using Transcription-Mediated Amplification (TMA)


This presentation uses HPV genotyping in a case of an abnormal pap test using TMA. It will include a discussion of how to design primers, the enzymes involved in TMA, and how it differs from PCR.

- Describe the steps of transcription-mediated amplification including which enzymes catalyze each step.

- Identify the distinctions between TMA from PCR, and determine how that changes primer design and interpretation of results.

Speaker: Jeffrey Gagan, MD, PhD Brigham & Women’s Hospital


Oligo Hybridization-Based Methodology: Microarray in the Infectious Diseases Diagnostic Laboratory


The case will cover the methodology behind oligo hybridization-based microarray assay, discuss an example of a microarray assay used in the infectious diseases diagnostic laboratory, and describe its impact on patient care.

- Review the basic concepts of microarray methodology.

- Describe the application of microarray methodology in the infectious diseases diagnostic laboratory.

Speaker: Sophie Arbefeville, MD University of Minnesota Medical Clinic

Triplet Repeat-Primed PCR for Detection of Trinucleotide Repeat Expansion


This case will provide an overview of PCR-based sizing analysis for the diagnosis of trinucleotide repeat disorders such as Huntington disease and Fragile X. Relative advantages and disadvantages of the method in comparison with historic approaches will be discussed.


- Describe the principles underlying triplet repeat-primed PCR for trinucleotide repeat sizing and compare with other sizing methodologies.

- Discuss the advantages and disadvantages of triplet repeat-primed PCR.

Speaker: Kristy Crooks, PhD University of Colorado


BCR-ABL1 Testing in CML


This case will discuss the clinical context, methods, interpretation, and clinical implications of BCR-ABL1 fusion detection in the setting of chronic myelogenous leukemia. Reverse transcriptase real time PCR will be discussed in detail.


- Describe how amplicon specificity is achieved using real time RT-PCR.

- Explain the purpose of an international scale value in interpretation of BCR-ABL1 quantitative results.

Speaker: Anthony N. Snow, MD University of Iowa Hospitals & Clinics


One-Step Wonders: Sample to Answer PCR in Infectious Diseases 

This case will describe the changing landscape of PCR for infectious disease diagnostics emphasizing the potential and pitfalls of sample to answer testing.


- Develop awareness of the role of sample to answer testing.

- Describe pitfalls associated with sample to answer testing.

Speaker: Kevin Alby, PhD Hospital of the University of Pennsylvania


1 in 160 Billion?  So You are Saying There is a Chance...


This case will illustrate the clinical applications of short tandem repeat analysis for identity testing as well as discuss the technical caveats of this assay that may limit the analytical sensitivity of this assay in some cases.

- List the clinical applications for identity testing by STR analysis.

- Describe the technical caveats with STR analysis.

SpeakerAnnette Kim, MD, PhD Brigham & Women\'s Hospital

Next-Generation Sequencing in AML


This case will review the use of next-generation sequencing to detect single nucleotide variants in acute myeloid leukemia (AML) and how genomic data is integrated in prognostication and sub classification of AML.

- Describe the prognostic significance of commonly mutated genes in acute myeloid leukemia.

- Describe the mechanism, advantages, and limitations of Ion Torrent next-generation sequencing.

Speaker: Jennifer Dunlap, MD Oregon Health & Science University


Difficult to Sequence Areas of the Genome


Next-generation sequencing technologies are now commonly used to clinically interrogate the entire genome or targeted regions of the genome. However, the underlying genome architecture poses significant challenges in the sequencing and analysis of genomic data which results in “holes” in the genome and cannot be addressed due to technical limitations of the current technologies. This case will present an overview of the current challenges and alternative techniques to address medically important but technically challenging regions of the genome.

- Describe the typical areas of the genome that present challenges with NGS based capture, sequencing and/or analysis.

- Identify the appropriate alternative applications to address such challenges.

Speaker: Avni Santani, PhD Children\'s Hospital of Philadelphia

Case Studies of Genomic Rearrangements Detected by DNA Next-Generation Sequencing: Modern Day ALKemy


Case studies involving genomic rearrangement of the ALK locus will be presented to illustrate the sequencing and bioinformatic challenges in detecting genomic rearrangements by direct next-generation sequencing (NGS) of DNA. The advantages and disadvantages of DNA-NGS, RNA-Seq, IHC, and FISH testing will be specifically addressed. The FDA-approved companion diagnostic FISH assay for crizotinib will be included in the discussion.

- Identify the pitfalls and challenges in identifying and interpreting genomic rearrangements from DNA NGS data.

- Compare and contrast the available techniques for identifying genomic rearrangements involving the ALK locus.

Speaker: Jason Rosenbaum, MD Hospital of the University of Pennsylvania


Multiplex Fusion Detection Using RNA Anchored Multiplex PCR (AMP) for Solid Tumors


This case will discuss clinical context, conceptual method details, advantages, and interpretation of anchored multiplex PCR for multiplex fusion detection in the context of a clinical case.

- Describe advantages of anchored multiplex PCR over previous methods including fluorescent in situ hybridization.

- Describe the uses of a molecular barcode and unique start sites as applied in anchored multiplex PCR.

Speaker: Anthony N. Snow, MD University of Iowa Hospitals & Clinics

Single-Nucleotide Polymorphism (SNP) Arrays and Array Comparative Genomic Hybridization for the Detection of Copy-Number and Copy-Neutral Genetic Abnormalities


This case will compare the utilization, advantages, and disadvantages of SNP arrays and aCGH for detecting copy-number abnormalities (e.g., duplication, deletion) and copy-neutral abnormalities (e.g., loss of heterozygosity, uniparental disomy).

- Describe the principles underlying SNP array and aCGH methodologies.

- Compare the relative advantages and disadvantages of the two methodologies in different genetic or clinical scenarios.

Speaker: Kristy Crooks, PhD University of North Carolina at Chapel Hill

Copy-Number Determination in Cancer Samples Using Next-Generation Sequencing Assays


This case will provide background, methods and practical examples of copy-number variation (CNV) detection as a part of next-generation sequencing (NGS) oncology profiling. Pros and cons of this methodology for CNV detection compared with other methodologies (e.g. FISH) will be discussed.

- Describe how NGS data from cancer specimens may be utilized to produce clinically applicable copy-number information.

- Compare the pros and cons of NGS oncology copy-number results in comparison with other methods, especially FISH.

Speaker: Jeremy Segal, MD, PhD University of Chicago

Liquid Biopsies- Just Go With the Flow




This case will highlight a common clinical application and one methodology for liquid biopsies. The case will include a discussion of some of the terminology, pre-analytic, analytic, and post-analytic issues surrounding liquid biopsies.

- List the appropriate clinical scenarios in which liquid biopsies are currently used and potential future applications.

- Describe the specimen limitations that are inherent to liquid biopsies.


Speaker: Annette S. Kim, MD, PhD Brigham & Women\'s Hospital




Molecular Weapons to Avoid Invasive Testing: NIPT




This case will demonstrate the usefulness of non-invasive prenatal testing (NIPT) using cell- free DNA in the clinical laboratory. The case will also discuss the technical procedures as well as the clinical utility and pitfalls of the test.

- Explain the procedures involved in NIPT.

- Evaluate the usefulness and pitfalls of the technique.


Speaker: Roberta Sitnik, PhD Hospital Israelita Albert Einstein

Individual topic purchase: Selected
American Board of Pathology
Self-Assessment Module: 4.50
American Medical Association
Continuing Medical Education: 4.50
American Society for Clinical Pathology
CMLE: 4.50
This seminar is no longer available for purchase.